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    AmpliScribe T7 High Yield Transcription Kit/高产体外转录试剂盒

    货号
    AS3107
    售价
    3931
    规格
    50 Reactions
    CAS号
  • 产品简介
  • 相关产品
  • 产品信息

    货号

    产品名称

    规格

    价格/

    ASF3507/ASF3257

    AmpliScribeT7-Flash Transcription Kit/AmpliScribe T7快速体外转录试剂盒

    50T/25T

    4253/2831

    AS3107

    AmpliScribe T7 High Yield Transcription KitAmpliScribe T7高产转录试剂盒

    50T

    3191

    DS010910/DS010925

    DuraScribe T7 Transcription Kit/核酸酶耐受体外转录试剂盒

    10T/25T

    4567/8466

    ASB71110

    AmpliScribe T7-Flash Biotin-RNA Transcription Kit 生物素RNA体外转录试剂盒

    10T

    5501

    AS3107

    AmpliScribe T7 High Yield Transcription Kit/高产体外转录试剂盒

    50T

    3931

    产品简介

          AmpliScribe™T7高产转录试剂盒特殊配方使得用户能够从体外转录反应中获得最大可能的RNA产量。利用1μg对照模板在标准的2小时,20-μL AmpliScribe反应体系中,将掺入反应体系中高达90%的NTP,并产生高达150μg的RNA。

          The AmpliScribe™ T7 High Yield Transcription Kit is specially formulated to enable users to obtain the maximum possible yields of RNA from an in vitro transcription reaction. The standard 2-hour, 20-µL AmpliScribe reaction will incorporate up to 90% of input NTPs and produce up to 150 µg of RNA from 1 µg of the control template. These yields are made possible by the high-performance properties of the AmpliScribe T7 enzyme preparations.

          AmpliScribe Kits produce exceptionally high yields of either long or short transcripts. The standard AmpliScribe reaction can be scaled up to produce milligram amounts of RNA. In addition, an AmpliScribe reaction can be readily modified to prepare fluorescent-1, biotinylated-2, or digoxigenin-3labeled RNA. Produce high yields of high quality RNA quickly and easily.

    Formerly from Epicentre Synthesize long and short RNA with one kit

    Produce >20 times more RNA than conventional or "homemade" in vitro transcription kits

    Components:


    AmpliScribe T7 Enzyme Solution, 

    100 mM ATP, CTP, GTP, and UTP Solutions, 

    AmpliScribe 10X Reaction Buffer, 

    100 mM Dithiothreitol (DTT), 

    RiboGuard™ RNase Inhibitor, 

    RNase-Free Water, 

    Control Template DNA, and RNase-Free DNase I

    参考文献:

    DeLong, E.F. et al., (1999) Appl. and Environ. Microbiol., 56, 5554.

    Hoffman, L.M. and Johnson, M.G. (1994) BioTechniques 17, 372.

    Kaplan, E.D. et al., (1996) Epicentre Forum, 3 (2), 1.

    Molecular Cloning - A Laboratory Manual, Third Edition, 2001. CSHL Press. pp 7.27 –7.34. J. Sambrook and D. Russell.