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TwistAmp nfo

货号
TANFO02KIT
售价(元)
6560
规格
96T
CAS号
TANFO02KIT
  • 产品简介
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  • TwistAmp® 核酸扩增产品,能够在15分钟内进行常温下的单分子核酸检测该技术对硬件设备的要求很低,特别适合用于体外诊断、兽医、食品安全、生物安全、农业等领域。TwistAmp nfo用于末端检测,检测DNA扩增反应. 末端三明治法快速检测,如侧向流试纸法.除基本组分之外, nfo核酶,和适合是LF 探针 形成新的聚合酶增强底物,无需任何仪器。本品可通过加入exo探针作为Amp exo kit的替代物,进行荧光定量检测,由于核酶延缓动态反应速度,可用于末端电泳检测。

     Basic Information:

    RPA

    1) Primers must be 30-35 bases

    2) Works best at constant temperature (37-39°C)

    3) Amplicons of 80-400bp are preferred

    4) TwistAmp® LF Probe or TwistAmp® exo Probe 1  required - see overleaf

    Set-up (single-plex)2

    1) Prepare reaction mix in 1.5ml tube:

    Primer A (10μM)    2.1 μl

    Primer B (10μM)    2.1 μl

    TwistAmp® LF Probe (10μM)      0.6 μl

    Rehydration Buffer   29.5 μl

    Template and dH2O   13.2 μl

    (Total Volume    47.5 μl)

    Vortex and spin briefly

    2) Add reaction mix to freeze-dried reaction. Pipette to mix.

    3) Add 2.5 μl of 280mM MgAc (supplied) and mix well to start reaction.

    4) Incubate at 37-39°C for 20-40 minutes.

    4b) For low template copy number,remove strip after 4 minutes, vortex &spin briefly, replace in heating device.

    5) For analysis by lateral flow, dilute reacion products 1/50 with PBST and load 10 μl onto the sample pad of appropriate

    strip (eg Milenia® HybriDetect strips).

    6) Place strip in PBST running buffer.

    WARNING: RPA REACTIONS START AT ROOM TEMPERATURE AS SOON AS

    MAGNESIUM IS ADDED.

    温馨提示:不可用于临床治疗。